CHI is the Compositae perennial herbs,leaves and roots to use its succulent cultivation was to soften the buds are edible ball,is the emerging specialty vegetables.CHI Nailao of the poor,if the growing field of stagnant water is easily aroused Mei Lan.At present,the CHI study focused on cultivation,in vitro culture in its report less.This test from the hormone,and other aspects of the CHI regeneration system for in vitro studies,the results are as follows:
(1) CHI four explants to do A and B of the implant treatment to good effect in the C and D do explants of treatment.
(2) CHI dedifferentiation easy.E or F-plus can be more rapid start-up CHI explants of dedifferentiation.However,its E-plus callus to good effect on the differentiation of F-plus results.Canadian F-induced callus easy to divide a large number of roots.In the E-plus,again F-induced Chicory explants to better-performing division.Research shows that when the F to 3.0 mg / L,G to 0.3 mg / L at the CHI callus induction and differentiation in the best effect.
(3) the use of different varieties of CHI callus differentiation effect in the study found,H callus variety of differentiation better than I varieties.
(4) The trial of three kinds of basic use of the medium CHI adventitious buds to the direct induction.As the medium types and concentrations of inorganic elements of the reduced CHI adventitious buds on the induction rate be reduced:M medium-induced effects of the best,S medium Secondly,the W medium did not induce adventitious buds.
(2) do not add any hormones in the medium on the basis of M,CHI-induced adventitious buds in different parts of abilities among larger:J and K can be induced adventitious buds,and L can not be induced adventitious buds and,R induction P higher than the rate of the induction rate.
3 CHI tissue culture take root more easily,but high concentrations inhibit its roots.Q induced by the effect of rooting better than R,the best medium for rooting:M
